Subgingival plaque samplings were gathered from the disto-buccal gingival crevice of the left upper central incisors (U1) and the left lower central incisors (L1), and from the mesio-buccal gingival crevice of the left upper first molars (U6) and the left lower first molars (L6), at two different times: at baseline, 2 weeks before appliance removal (T1); and 3 months after appliance removal (T2).

Of the sampling of all assessed sites, the highest probability for simultaneously detecting the tested pathogens was found in respect to the combination of supra- and subgingival plaque samples 1994-08-01 Conclusion: Salivary analysis might be discussed as a potential alternative to subgingival plaque sampling for microbiologic analysis in both AgP and CP. Discover the world's research. Subgingival plaque sampling and clinical recording (at baseline) and scaling and root planing was performed. Two weeks later the selected periodontal sites were submitted to one of the following treatments: Irrigation with a hydroalcoholic solution of propolis extract twice a week for 2 weeks (group A); irrigation with a placebo twice a week for 2 weeks (group B); or no additional treatment Before and immediately after treatment, subgingival plaque samples were taken from interdental sites with 3 to 5 mm probing depth (PD) at 2 test teeth and 2 positive control teeth. To evaluate the influence of sampling on the microflora, plaque samples were also taken twice at 2 teeth without therapy with PD of 3 to 5 mm (negative control).

Subgingival plaque sampling

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(2017) Okada et al. Journal of Oral Science. Quantitative analysis of periodontal bacteria is considered useful for clinical diagnosis, evaluation and assessment of the risk of periodontal disease. BACKGROUND: The chewing stick, the miswak, is used in many developing countries as the traditional means for oral hygiene.

been used to sample subgingival plaque. The tech-nique used may influence the outcome of the mi-crobiological analysis.5,6 Also, the number of sample sites is essential, especially when samples are pooled.7 Microbial subgingival sampling is time con-suming and involves the selection of sampling sites, isolation and drying of these sites, and

Autores: Diana-Maria Krigar Localización: Journal of periodontology, ISSN 0022-3492, Vol. 78, Nº. 1, 2007, págs. 72-78 Distribution of3-Hydroxy iC17:0 in Subgingival Plaque and Gingival Tissue Samples: Relationship to Adult Periodontitis FRANKC.

Subgingival plaque sampling


Subgingival plaque sampling

Jörg K. Krieger Section of Periodontology, Department of Conservative Dentistry, Clinic for Oral, Dental and Maxillofacial Diseases, University Hospital Heidelberg 2011-01-05 · A subgingival plaque sample was obtained as follows. After supragingival plaque was carefully removed and sampling sites were isolated with cotton rolls and air-dried, a subgingival plaque sample was collected with 2 sterile paper points per site. Plaque sampling for DNA test was performed at the baseline, 1 week after professional tooth cleaning, and after 3 weeks of either miswak or toothbrush use. Identification and quantification of microbial species were performed by the checkerboard method, using whole genomic, digoxigenin-labelled DNA probes. (2017) Okada et al. Journal of Oral Science. Quantitative analysis of periodontal bacteria is considered useful for clinical diagnosis, evaluation and assessment of the risk of periodontal disease.

for example displays different bacterial profiles than plaque samples. 1 May 2018 Twenty-one subgingival plaque samples were collected from three patients with chronic periodontitis (ChP), three patients with aggressive  In this investigation, we determined whether the association between BANA hydrolysis and spirochetes could be obtained in pooled subgingival plaque samples  6 Jan 2021 Subgingival plaque samples from 587 dogs were included in the study. Breed size and age are potential risk factors for periodontitis and  In this report over 400 subgingival plaque samples taken from over 110 patients were examined microscopically and culturally for 30 bacterial parameters. Abstract. Background: The collection of subgingival plaque samples with paper points is time-consuming and accident-sensitive. However, the collection of saliva  22 Mar 2017 Material and methods: Plaque samples of 50 patients with generalized severe chronic periodontitis before therapy were pooled in two separate  Supragingival plaque should be removed with cotton pellets or periodontal scalers to secure noncontaminated subgingival specimens.
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Subgingival plaque sampling

Analysis was performed using a commercially available polymerase chain reaction test for 11 periodontal pathogens. A Study to Evaluate Sampling Methods for Subgingival Plaque The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government.

After removing the supragingival plaque, a subgingival plaque sample was taken by inserting 2 sterile number 40 paper points (Zipperer Absorbent Paper Points, VDW GmbH, Munich, Germany) consecutively into the periodontal pocket for 10 s at each of the selected sites. 2015-04-01 · Indeed, due to the close contact of plaque with cells from the periodontal tissues and crevicular fluid, human DNA can be incorporated during subgingival plaque sampling.
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the subgingival microbiota, pathogens, and plaque [12]. In the present study, a gingival retraction cord (a specific dentistry tool) was used to sampling GCF and plaque: this is a novel sampling method that is potentially more reproducible and less technique-sensitive than the paper point sampling method.

Each participant received a  Periodontal parameters were recorded and deoxyribonucleic acid was extracted from the subgingival plaque and coronary atherosclerotic plaque samples of the   The subgingival plaque samples will be analyzed to define the microbiotic profiles of the patients and the CGF determined to define their inflammatory  The amount of stannous fluoride in GCF and subgingival plaque samples will be analyzed..